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Biomaterial field:





Medical device field:LYDEX      


Controlling the degradation of an oxidized dextran-based hydrogel independent of the mechanical properties. Carbohydr Polym. 2019 Jan 15;204:131-141. doi: 10.1016/j.carbpol.2018.09.081. Epub 2018 Oct 3.
Dextran oxidized by a malaprade reaction shows main chain scission through a maillard reaction triggered by schiff base formation between aldehydes and amines. Journal of Polymer Science Part A: Polymer Chemistry / Volume 54, Issue 14. First published: 16 March 2016
Self-degradation of tissue adhesive based on oxidized dextran and poly-l-lysine Carbohydrate Polymers Volume 113, 26 November 2014, Pages 32–38
Low cytotoxic tissue adhesive based on oxidized dextran and epsilon-poly-l-lysine. J Biomed Mater Res A. 2014 Aug;102(8):2511-20. doi: 10.1002/jbm.a.34923. Epub 2013 Aug 30.
Medical Adhesive using the Food Additives ( Journal of The Adhesion Society of Japan, 2013 Volume 49 Issue 8 Pages 293-304
Self-Degradable Bioadhesive. 2007, Key Engineering Materials, 342-343, 713


The effective control of a bleeding injury using a medical adhesive containing batroxobin." Biomed Mater. 2014 Apr;9(2):025002. doi: 10.1088/1748-6041/9/2/025002. Epub 2014 Jan 31.
Hemostatic effect of new surgical glue in animal partial nephrectomy models.  Urology. 2013 May;81(5):1095-100. doi: 10.1016/j.urology.2013.01.002. Epub 2013 Feb 20.
▶Evaluation of Wound Closure and Antihemorrhagic Efficiency of Medical Adhesive Composed of Aldehyded Dextran and Poly(L-lysine). Biomaterials Research 15(3) : 129-135 (2011)


Creation of a uniform pleural defect model for the study of lung sealants. J Thorac Cardiovasc Surg. 2007 Jul;134(1):145-51.

Anti-adhesion material

Prevention of retrosternal adhesion by novel biocompatible glue derived from food additives. J Thorac Cardiovasc Surg. 2013 Nov;146(5):1232-8. doi: 10.1016/j.jtcvs.2013.02.001. Epub 2013 Apr 10.
Novel powdered anti-adhesion material: preventing postoperative intra-abdominal adhesions in a rat model. Int J Med Sci. 2013;10(4):467-74. doi: 10.7150/ijms.5607. Epub 2013 Mar 5.
Novel biodegradable powder for preventing postoperative pleural adhesion. J Surg Res. 2013 Jan;179(1):e13-9. doi: 10.1016/j.jss.2012.01.056. Epub 2012 Apr 1.

Wound care dressings with endoscope

Efficacy of a novel endoscopically deliverable muco-adhesive hemostatic powder in an acute gastric bleeding porcine model. PLoS One. 2019 Jun 11;14(6):e0216829. doi: 10.1371/journal.pone.0216829. eCollection 2019.

Drug Delivery System

Aldehyded Dextran and ε-Poly(L-lysine) Hydrogel as Nonviral Gene Carrier. Stem Cells Int. 2013;2013:634379. doi: 10.1155/2013/634379. Epub 2013 Aug 21.
Topical application of a biodegradable disc with amiodarone for atrial fibrillation. Ann Thorac Surg. 2011 Mar;91(3):734-9. doi: 10.1016/j.athoracsur.2010.10.022.


Development of a new tissue-engineered sheet for reconstruction of the stomach. Artif Organs. 2009 Oct;33(10):818-26. doi: 10.1111/j.1525-1594.2009.00808.x. Epub 2009 Oct 16.
Biodegradable Adhesive (LYDEX) with Hydroxyapatite Granules. 2007, Key Engineering Materials, 361-363, 575

Ophthalmology field

An ex vivo model for suture-less amniotic membrane transplantation with a chemically defined bioadhesive.. Curr Eye Res. 2012 May;37(5):372-80. doi: 10.3109/02713683.2012.663853. Epub 2012 Mar 29.
Novel sutureless keratoplasty with a chemically defined bioadhesive. Invest Ophthalmol Invest Ophthalmol Vis Sci. 2009 Jun;50(6):2679-85. doi: 10.1167/iovs.08-2944. Epub 2009 Jan 10.
Sutureless amniotic membrane transplantation for ocular surface reconstruction with a chemically defined bioadhesive. Biomaterials. 2008 Jul;29(19):2923-31. doi: 10.1016/j.biomaterials.2008.03.027. Epub 2008 Apr 15.

Orthopedics field

Strong adhesiveness of a new biodegradable hydrogel glue, LYDEX, for use on articular cartilage. J Appl Biomater Funct Mater. 2013 Dec 16;11(3):e180-6. doi: 10.5301/JABFM.5000164.


Sustained release of vancomycin from a new biodegradable glue to precent methicillin-resistant Ataphylococcus aureus graft ingection. Interact Cardiovasc Thorac Surg. 2010 Jul;11(1):52-5. doi: 10.1510/icvts.2010.232447. Epub 2010 Apr 1.

Gastrointestinal surgery field


Life science field:Antifreeze polyamino acid

Cell preservation

Cryopreservation of bovine somatic cells using antifreeze polyamino-acid (carboxylated poly-l-lysine). Cryobiology. 2017 Feb 1. pii: S0011-2240(16)30436-9. doi: 10.1016/j.cryobiol.2017.01.010. 
Long-term cryopreservation of human mesenchymal stem cells using carboxylated poly-l-lysine without the addition of proteins or dimethyl sulfoxide. J Biomater Sci Polym Ed. 2013 Aug;24(12):1484-97. doi: 10.1080/09205063.2013.771318. Epub 2013 Feb 22.
Effective vitrification of human induced pluripotent stem cells using carboxylated ε-poly-l-lysine. Cryobiology. 2011 Oct;63(2):76-83. doi: 10.1016/j.cryobiol.2011.05.003. Epub 2011 May 20.
▶ Polyampholytes as cryoprotective agents for mammalian cell cryopreservation. 2010;19(6):691-9. doi: 10.3727/096368910X508780. Epub 2010 Jun 3.
Polyampholytes as low toxic efficient cryoprotective agents with antifreeze protein properties. Biomaterials. 2009 Sep;30(27):4842-9. doi: 10.1016/j.biomaterials.2009.05.025. Epub 2009 Jun 9.

Chondrocyte sheet preservation

Development of a novel vitrification method for chondrocyte sheets. BMC Biotechnol. 2013 Jul 25;13(1):58. [Epub ahead of print]

Myoblast sheet presevation


Cell encapsulation and cryostorage in PVA-gelatin cryogels: incorporation of carboxylated ε▶L-lysine as cryoprotectant. J Tissue Eng Regen Med. 2012 Apr;6(4):280-90. doi: 10.1002/term.431. Epub 2011 Jun 27.

Fertilized egg preservation (infertility treatment)

Successful vitrification of pronuclear-stage pig embryos with a novel cryoprotective agent, carboxylated ε-poly-L-lysine. PLoS One. 2017 Apr 27;12(4):e0176711. doi: 10.1371/journal.pone.0176711. eCollection 2017. 
▶Development of a Low Toxicity and Completely Serum-Free Vitrification System Combining a Cryo-Nano-Hole Vitrification Container with Antifreeze Polyamino-acid (Carboxylated Poly-L-Lysine) for assisted reproductive technology (ART). J. Reprod. Engineer. 2011; Vol. 14 No. 2: 13-36.

Sperm preservation (infertility treatment)

Mouse germ cell preservation

Bovine germ cell preservation

Porcine germ cell preservation

Freezing mechanism

Antifreeze effect of carboxylated ε-poly-L-lysine on the growth kinetics of ice crystals. J Phys Chem B. 2014 Aug 28;118(34):10240-9. doi: 10.1021/jp507697q. Epub 2014 Aug 18.

Drug Delivery System

Protein cytoplasmic delivery using polyampholyte nanoparticles and freeze concentration. Biomaterials. 2014 Aug;35(24):6508-18. doi: 10.1016/j.biomaterials.2014.04.030. Epub 2014 May 10.

Life science fieldPolyphenols derived from green tea

Skin fibroblasts

Epigallocatechin-3-gallate regulates cell growth, cell cycle and phosphorylated nuclear factor-κB in human dermal fibroblasts. Acta Pharmacol Sin. 2011 May;32(5):637-46. doi: 10.1038/aps.2011.17. Epub 2011 Apr 25.
In vitro antifungal activity of epigallocatechin 3-O-gallate against clinical isolates of dermatophytes.  Yonsei Med J. 2011 May;52(3):535-8. doi: 10.3349/ymj.2011.52.3.535.

Skin tissue

Tissue bank (transplant)

Graft-versus-host disease

Attenuation of murine graft-versus-host disease by a tea polyphenol. Cell Transplant. 2012;21(5):909-18. doi: 10.3727/096368911X623934. Epub 2012 Feb 13.


Epigallocatechin-3-gallate protects kidneys from ischemia reperfusion injury by HO-1 upregulation and inhibition of macrophage infiltration. Transpl Int. 2011 May;24(5):514-22. doi: 10.1111/j.1432-2277.2011.01224.x. Epub 2011 Feb 3.

Peripheral nerve

Storage and allogeneic transplantation of peripheral nerve using a green tea polyphenol solution in a canine model. J Brachial Plex Peripher Nerve Inj. 2010 Nov 28;5:17. doi: 10.1186/1749-7221-5-17.
Peripheral nerve allografts stored in green tea polyphenol solution. Transplantation. 2005 Mar 27;79(6):688-95.

Vascular smooth muscle cells

Selective inhibitory effect of epigallocatechin-3-gallate on migration of vascular smooth muscle cells. Molecules. 2010 Nov 19;15(11):8488-500. doi: 10.3390/molecules15118488.

Aortic smooth muscle cells

Human saphenous vein

Human fibrosarcoma

Microvascular endothelial cells


Oral pretreatment with a green tea polyphenol for cardioprotection against ischemia-reperfusion injury in an isolated rat heart model. J Thorac Cardiovasc Surg. 2011 Feb;141(2):511-7. doi: 10.1016/j.jtcvs.2010.04.016. Epub 2010 May 23.


Articular cartilage



Human stem cells

Tarsal fibroblasts


Development of epigallocatechin gallate-eluting polymeric stent and its physicochemical, biomechanical and biological evaluations. Biomed Mater. 2009 Aug;4(4):044104. doi: 10.1088/1748-6041/4/4/044104. Epub 2009 Jul 8.


Liver cells

reservation of porcine hepatocytes in three-dimensional bioreactor at room temperature using epigallocatechin-3-gallate. Tissue Eng Part C Methods. 2009 Sep;15(3):345-53. doi: 10.1089/ten.tec.2008.0483.

Wound dressing

Enhanced wound healing by an epigallocatechin gallate-incorporated collagen sponge in diabetic mice. Wound Repair Regen. 2008 Sep-Oct;16(5):714-20. doi: 10.1111/j.1524-475X.2008.00422.x.


Enhanced antitumor activities of (-)-epigallocatechin-3-O-gallate fatty acid monoester derivatives in vitro and in vivo. Biochem Biophys Res Commun. 2008 Dec 26;377(4):1118-22. doi: 10.1016/j.bbrc.2008.10.128. Epub 2008 Nov 5.


Time-dependent intracellular trafficking of FITC-conjugated epigallocatechin-3-O-gallate in L-929 cells. Bioorg Med Chem. 2008 Nov 15;16(22):9652-9. doi: 10.1016/j.bmc.2008.10.009. Epub 2008 Oct 7.

Mammalian tissue

Non-frozen preservation of mammalian tissue using green tea polyphenolic compounds. Biomed Mater. 2006 Mar;1(1):R18-29. doi: 10.1088/1748-6041/1/1/R03. Epub 2006 Mar 21. Review.

Lymphocyte culture

Rat aortic tissue transplantation

Cell growth inhibition

Hibernation, reversible cell growth inhibition by epigallocatechin-3-O-gallate. J Biotechnol. 2007 Jan 20;127(4):758-64. Epub 2006 Sep 22.

Fungal infection

Antifungal susceptibility of epigallocatechin 3-O-gallate (EGCg) on clinical isolates of pathogenic yeasts. Biochem Biophys Res Commun. 2006 Aug 25;347(2):401-5. Epub 2006 Jun 15.

Ischemia/reperfusion injury

Rat abdominal aortic aneurysm

Prevention of abdominal aortic aneurysm progression by oral administration of green tea polyphenol in a rat model. J Vasc Surg. 2016 Jul 26. pii: S0741-5214(16)30419-0. doi: 10.1016/j.jvs.2016.06.003. 
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