StemCell Keep

StemCell Keep

Stem Cell Keep is a vitrification cryopreservation solution for primate ES/iPS cells. optimized to maintain high vitrification capacity and low cytotoxicity.

※ For research use only. Not for other uses.

※ Stem Cell Keep is sold by Funakoshi Co., Ltd.

 

Feature

• It does not contain DMSO, which is known to affect differentiation, or animal protein components.

• The colonies can be cryopreserved as they are by the vitrification method.

• Stem cells can be cryopreserved while maintaining their differentiation potential.

• With our newly developed cryoprotectant that offers high safety, superior cell protection, and excellent glass-state maintenance properties, we have achieved more efficient cryopreservation of ES/iPS cell colonies.

• When used according to protocol, 100 vials can be stored.

• Aseptic testing confirms that there is no contamination of bacteria, fungi, or mycoplasma.

• This product can be stored stably at 4°C for a long period of time (expiration date is 2 years).

Preservation of primate ES/iPS cells and vitrification freezing methods

Comparison

Examples

Outline operation

1. Prepare liquid nitrogen in a clean bench.

2. Primate ES / iPS cells are detached in colony condition in a detachment solution (0.25% trypsin/1mg/ml collagenase IV/PBS solution).
• At this time, a 60 mm dish can be used to freeze confluent cells to 1-5 vials.

3. Centrifuge the cells collected in step 2 and remove the medium. If multiple cells are to be frozen here, keep them ice-cooled and perform the following operations one at a time.

4. Add 200 μl of the product, pipet well, cover the vial, and immerse the entire vial in liquid nitrogen as soon as possible (within 1 minute).
• If the liquid remains clear, vitrification has been successful.

5. Store in a liquid nitrogen tank or - 130°C deep freezer.

1. Prepare medium warmed at 37°C and prepare the required number of centrifuge tubes containing 9 ml of medium.
• Thawing should be done one at a time.

2. Vials of cells frozen in this product are transported to a clean bench in liquid nitrogen in a Dewar bottle or similar container.

3. Open the lid of the removed vial and add 1 ml of warmed medium quickly and dissolve by pipetting.

4. Transfer the entire volume of 3 into a centrifuge tube containing 9 ml of medium prepared in 1, centrifuge and wash.

5. Seed and culture on feeder cells.